This NIH funded laboratory at the University of Maryland, Baltimore (UMB) investigates the molecular and cellular determinants of alloantigen specific tolerance using murine models of cardiac and pancreatic islet transplantation. Specific investigations involve the determination of mechanisms responsible for remodeling of secondary lymphoid organ structure, and how those mechanisms drive T cell migration, differentiation, and fate determination. The molecular focus of the investigations is directed to chemokines, chemokine receptors, cytokines, sphingosine receptors, lymphotoxin, and stromal fibers. Specific cellular elements include regulatory suppressive T cells, effector T cells, vascular and lymphatic endothelial cells, stromal cells, and dendritic cell subsets. Techniques include heavy reliance on in vivo animal models, migration and trafficking assays, real time imaging and advanced microscopy, multicolor flow and sorting, and a variety of molecular techniques for proteins and nucleic acids.
Successful candidates will have at a minimum a recent PhD in immunology or a highly related biomedical field, demonstration of success in publication and presentation, and familiarity or expertise in many of the techniques utilized by the lab.
Piao W, Xiong Y, Famulski K, Brinkman CC, Li L, Wagner C, Saxena V, Simon T, Bromberg JS. Regulation of T cell afferent lymphatic migration by targeting LTR-mediated non-classical NFB signaling. Nature Comm. 2018, 9:3020. doi: 10.1038/s41467-018-05412-0
Bromberg JS, Hittle L, Xiong Y, Saxena V, Smyth EM, Li L, Zhang T, Wagner C, Fricke WF, Simon T, Brinkman CC, Mongodin EF. Gut microbiota-dependent modulation of innate immunity and lymph node remodeling affects cardiac allograft outcomes. J Clin Invest Insight 2018, doi: 10.1172/jci.insight.121045
Xiong Y, Piao W, Brinkman CC, Li L, Kulinski JM, Olivera A, Cartier A, Hla T, Hippen K, Blazar B, Schwab SR, Bromberg JS. Sphingosine 1-phosphate (S1P) receptors differentially regulate CD4 T cell migration across afferent lymphatic endothelium. Science Immunology 2019, 4:eaav1263. doi: 10.1126/sciimmunol.aav1263
Piao W, Xiong Y, Li L, Saxena V, Smith KD, Hippen KL, Williams KM, Paluskievicz C, Willsonshirkey M, Stains JP, Abdi R, Blazar BR, Bromberg JS. Regulatory T cells condition endothelial cells for enhanced transendothelial migration. Cell Reports 2020, 30:1052-1062.
Lushen L, Willsonshirkey M, Zhang T, Xiong Y, Piao W, Saxena V, Paluskievicz C, Lee Y, Toney N, Cerel BM, Li Q, Simon T, Smith KD, Hippen KL, Blazar BR, Abdi R, Bromberg JS. The lymph node stromal laminin 5 shapes alloimmunity. J Clin Invest, in press.