Role at a Glance:
The Hargus lab at the Taub Institute and the Department of Pathology and Cell Biology at Columbia University Irving Medical Center is looking for a highly motivated and driven postdoctoral research scientist with a strong interest in stem cell biology, neural development, and neurodegenerative diseases. We use differentiated human-induced pluripotent stem cells to identify mechanisms leading to neuronal degeneration in tauopathies including frontotemporal dementia and Alzheimer’s disease. This research project will include the application of in vitro disease modeling techniques such as neuron-microglia co-cultures and single-cell RNA sequencing as well as transplantation of differentiated stem cells into mice. The position is available full-time. Career development including attendance at conferences is strongly supported.
At Columbia University, we stand together because diverse experiences, perspective, and values enrich every dimension of our work. All qualified applicants will receive consideration for employment independent of race, ethnicity, religion, creed, sexual orientation, gender identity or expression, national origin, age, disability, etc. or any other factor which cannot be used as a basis for employment decision.
Job Function:
- Culture and differentiation of induced pluripotent stem cells into neurons and glial cells
- In vitro assay on differentiated cells
- Transplantation of iPSC-derived neurons into the brains of immunocompromised mice
- Histology of grafts followed by confocal imaging and quantification
- Microdissection of grafted human neurons followed by snRNA-seq
Lab Mission/Purpose:
Our lab applies human induced pluripotent stem cells (iPSCs) to model neurodegenerative diseases, with a special emphasis on tauopathies including frontotemporal dementia and Alzheimer’s disease.Current studies within the lab focus on elucidating the mechanisms of disturbed function in patient neurons. We apply cell stress, seahorse and metabolomics assays, bulk and single cell sequencing, and co-culture assays using iPSC-derived neurons and glial cells including astrocytes and microglia to characterize cell-intrinsic disease phenotypes as well as non-cell-autonomous mechanisms of disease development. Furthermore, we transplant patient and control iPSC-derived neural cells into the brains of immunocompromised mice followed by histology and snRNA-seq of microdissected grafts. These studies are complemented by snRNA-seq studies on postmortem brain tissue from patients with tauopathies carrying the same disease-associated mutations as our iPSCs.
http://columbianeuroresearch.org/taub/res-featured-5-2019.html
Qualifications
- Minimum Skills Required:
- Experience with cell culture and common molecular techniques.
- Minimum Education Requirements:
- PhD degree in sciences/neuroscience-related fields
- Preferred Qualifications:
- Experience with stem cell culture and differentiation
Application Instructions
Hiring Salary Range: 65,000-$65,000
The salary of the finalist selected for this role will be set based on a variety of factors, including but not limited to departmental budgets, qualifications, experience, education, licenses, specialty, and training. The above hiring range represents the University’s good faith and reasonable estimate of the range of possible compensation at the time of posting.
Application Process
This institution is using Interfolio's Faculty Search to conduct this search. Applicants to this position receive a free Dossier account and can send all application materials, including confidential letters of recommendation, free of charge.
In order to apply please visit: http://apply.interfolio.com/121854
